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Oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients
Author(s) -
Blanc V,
O'Valle F,
Pozo E,
Puertas A,
León R,
Mesa F
Publication year - 2015
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12364
Subject(s) - tannerella forsythia , periodontitis , fusobacterium nucleatum , treponema denticola , aggregatibacter actinomycetemcomitans , prevotella intermedia , eikenella corrodens , medicine , porphyromonas gingivalis , actinomyces , microbiology and biotechnology , placenta , biology , bacteria , pregnancy , pathology , fetus , honeysuckle , alternative medicine , traditional chinese medicine , genetics
Objectives The objective of this study was to identify the DNA of oral bacteria in placental samples from women with and without periodontitis who had or had not had preterm births and/or low birthweight ( PB / LBW ) neonates. Methods Data were gathered from 57 puerperal women in relation to socio‐demographic, gynaecological, and periodontal variables and to placental histomorphology. Fifty‐seven biopsies, 28 from mothers with periodontitis, were taken aseptically from preterm placentas ( n = 36) and from full‐term placentas ( n = 21). Total DNA was extracted, and the presence of 15 oral bacteria was assessed using Nested‐ PCR . Results The placentas from women with periodontitis showed a higher prevalence of periodontopathogens compared to those from women without periodontitis ( P = 0.009). Samples showed low prevalences of Actinomyces israelii , Parvimonas micra and Tannerella forsythia . An association was found between Eikenella corrodens in placenta and periodontitis ( P = 0.002). The most ubiquitous bacterium, Fusobacterium nucleatum, was more prevalent in mothers with periodontitis and PB / LBW ( P = 0.033). Porphyromonas gingivalis, Treponema denticola, Prevotella intermedia and Aggregatibacter actinomycetemcomitans were not detected. Conclusions These results, along with previous findings, show that oral bacteria may be normally present in the placenta, however, the levels of certain oral pathogens in the placenta would highly depend on the mother's periodontal state.