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Laser therapy reduces gelatinolytic activity in the rat trigeminal ganglion during temporomandibular joint inflammation
Author(s) -
Desiderá AC,
Nascimento GC,
Gerlach RF,
LeitePanissi CRA
Publication year - 2015
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12330
Subject(s) - temporomandibular joint , orofacial pain , low level laser therapy , medicine , trigeminal ganglion , inflammation , saline , zymography , matrix metalloproteinase , anesthesia , endocrinology , pathology , laser therapy , surgery , laser , psychology , physics , sensory system , optics , cognitive psychology
Objectives To investigate whether low‐level laser therapy ( LLLT ) alters the expression and activity of MMP ‐2 and MMP ‐9 in the trigeminal ganglion ( TG ) during different stages of temporomandibular joint ( TMJ ) inflammation in rats. It also evaluated whether LLLT modifies mechanical allodynia and orofacial hyperalgesia. Materials and Methods Wistar rats (±250 g) were divided into groups that received saline ( SAL ) or complete Freund's adjuvant ( CFA , 50 μ l) in the TMJ , and that later underwent LLLT (20 J cm −2 ) at their TMJ or not (groups SAL , SAL + LLLT , CFA , and CFA + LLLT ). LLLT was applied on days 3, 5, 7, and 9 after SAL or CFA . Mechanical allodynia was evaluated on days 1, 3, 5, 7, and 10; orofacial hyperalgesia was assessed on day 10. Gelatin zymography and in situ zymography aided quantification of MMP s in the TG . Results Low‐level laser therapy abolished the reduction in the mechanical orofacial threshold and the increase in orofacial rubbing during the orofacial formalin test induced by CFA . LLLT also decreased the CFA ‐induced rise in the levels of MMP ‐9 and MMP ‐2 as well as the gelatinolytic activity in the TG . Conclusion Low‐level laser therapy could constitute an adjuvant therapy to treat temporomandibular disorders and prevent inflammation‐induced alterations in the levels of MMP ‐2 and MMP ‐9 and in the gelatinolytic activity in TG s.