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Characterization of mesenchymal progenitor cell populations from non‐epithelial oral mucosa
Author(s) -
Matsumura S,
Higa K,
Igarashi T,
Takaichi S,
Tonogi M,
Shinozaki N,
Shimazaki J,
Yamane Gy
Publication year - 2015
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12288
Subject(s) - mesenchymal stem cell , neural crest , homeobox protein nanog , biology , stem cell , progenitor cell , flow cytometry , stem cell marker , microbiology and biotechnology , pathology , embryonic stem cell , induced pluripotent stem cell , embryo , medicine , biochemistry , gene
Objectives The characteristics of cell populations extracted from oral mucosal non‐epithelial tissues and their ability to differentiate were evaluated in vitro as a potential source of cells for mandibular and corneal regeneration. Materials and methods Oral mucosal non‐epithelial cells ( OMNEC s) were extracted from tissue samples and were studied by flow cytometry and RT ‐ PCR . Cells differentiating into osteoblasts, adipocytes, chondrocytes, neurocytes, or keratocytes were characterized by RT ‐ PCR and cell staining. Results OMNEC s expressed CD 44, CD 90, CD 105, CD 166, and STRO ‐1 antigens, which are markers for mesenchymal stem cells. In addition, Oct3/4, c‐Myc, Nanog, KLF 4, and Rex, which are expressed by embryonic or pluripotent stem cells, were detected by RT ‐ PCR . Expression of CD 49d, CD 56, and PDGFR α , proteins closely associated with the neural crest, was observed in OMNEC s, as was expression of Twist1, Sox9, Snail1 and Snail2, which are early neural crest and neural markers. Specific differentiation markers were expressed in OMNEC s after differentiation into osteoblasts, adipocytes, chondrocytes, or keratocytes. Conclusions Populations of OMNEC s may contain both mesenchymal stem cells and neural crest origin cells and are a potential cell source for autologous regeneration of mandibular or corneal stroma.