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Original Research
Author(s) -
Elena Maria Varoni,
Erica Iasi,
G. Lodi,
Lia Rimondini,
Luigi De Nardo,
Lina Altomare
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12277
Subject(s) - citation , library science , original research , computer science
Objectives: Here, we propose the application of a novel film-like chitosan (CH) strips as\udoral patches to enhance wound healing and buccal drug delivery. The purpose is to\udimprove mucoadhesion in the oral environment, exploiting the well-known bioadhesive\udproperties of CH and the mechanical retention of strips, by means of their innovative\udhighly oriented micropattern of porosity.\udMethods: CH strips were produced by cathode electrodeposition. Morphological and\udphysical characterization was performed using Optic microscopy, Scanning Electron\udMicroscope (SEM) and swelling degree analyses. Cytocompatibility with primary human\udgingival fibroblasts (HGF) was verified by means of MTT assay and SEM images. Mucoadhesion\udwas tested using an ex vivo retention model, based on porcine buccal mucosa\udand DMA Q800 TA instrument.\udResults: CH strips displayed an oriented porosity, with pores of about 500 lm of diameter,\udand a random microporosity due to H2 bubble production during synthesis (20 lm).\udIn PBS (pH7.4), structures displayed a excellent water uptake, in few minutes. High cytocompatibility,\udafter primary HGF seeding onto oriented pores samples, was demonstrated.\udInteresting mucoadhesive properties were verified by ex vivo test.\udConclusion(s): Morphological, physical and biological characterizations showed as CH\udstrips are suitable bioadhesive device for buccal applications.\udRelevance: In perspective, these findings are relevant to produce drug delivery system for\udoral mucosa as well as to obtain mucoadhesive patches to enhance wound healing by\udmechanical protection