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Cell dynamics in H ertwig's epithelial root sheath and surrounding mesenchyme in mice irradiated to the head
Author(s) -
Ide Y,
Nakahara T,
Nasu M,
Ishikawa H
Publication year - 2015
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12253
Subject(s) - mesenchyme , epithelium , dynamics (music) , microbiology and biotechnology , chemistry , cell , anatomy , biology , pathology , medicine , biochemistry , physics , acoustics
Objective To investigate the mechanisms that cause damage to root formation as a result of irradiation to the mouse head, morphological changes in molar dental roots and cell dynamics in H ertwig's epithelial root sheath ( HERS ), and surrounding mesenchymal tissue were examined. Materials and Methods To perform the experiments, 5‐day‐old C 57 BL /6 mice were randomly divided into three groups: the control group (0 Gy) and irradiated groups (10 and 20 Gy). Micro‐ CT analysis, HE staining, immunohistochemistry analysis, and TUNEL assay were then performed. Results Roots in irradiated mice were dose‐dependently shorter than those of control mice. Cells located outside the root dentin, with abnormal morphology in irradiated mice, were positive for an odontoblast marker. HERS fragmentation occurred earlier in irradiated mice than in control mice, and HERS was trapped by the calcified apical tissue. A dose‐dependent reduction in the number of proliferating cells within the apical dental pulp and periapical periodontal ligament surrounding HERS was observed in irradiated mice. Apoptotic cells in the dental pulp and periodontal ligament surrounding HERS were hardly seen. Conclusions These results indicate that the early disappearance of HERS and the proliferative suppression of the surrounding mesenchymal cells, which was induced by irradiation, caused dental root malformation.