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Retracted: IL ‐1 β ‐induced matrix metalloproteinase‐3 regulates cell proliferation in rat dental pulp cells
Author(s) -
Ozeki N,
Yamaguchi H,
Hiyama T,
Kawai R,
Nakata K,
Mogi M,
Nakamura H
Publication year - 2015
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12219
Subject(s) - apoptosis , matrix metalloproteinase , dna fragmentation , cell growth , pulp (tooth) , microbiology and biotechnology , chemistry , interleukin , cell , fragmentation (computing) , andrology , programmed cell death , biology , immunology , medicine , cytokine , pathology , biochemistry , ecology
Objective We previously reported that matrix metalloproteinase‐3(MMP‐3) accelerates wound healing following dental pulp injury. In this study, we tested the hypothesis that induction of MMP‐3 activity by interleukin‐1 β would promote proliferation and apoptosis of dental pulp cells. Materials and Methods Dental pulp cells were isolated from rat incisors and subjected to interleukin‐1 β . Matrix metalloproteinase‐3 m RNA and protein expression were assessed using reverse transcription–polymerase chain reaction and Western blotting, respectively. Matrix metalloproteinase‐3 activity was measured using fluorescence. Dental pulp cell proliferation and apoptosis were determined using enzyme‐linked immunosorbent assays ( ELISA ) for BrdU and DNA fragmentation, respectively. siRNA was used to reduce MMP‐3 transcripts in these cells. Results Treatment with interleukin‐1 β increased MMP‐3 mRNA and protein levels as well as its activity in dental pulp cells. Cell proliferation was also markedly increased, with no changes in apoptosis observed. Treatment with siRNA against MMP‐3 potently suppressed this interleukin‐1 β ‐induced increase in MMP‐3 expression and activity, and also suppressed cell proliferation but unexpectedly increased apoptosis in these cells ( P < 0.05). This siRNA‐mediated increase in apoptosis could be reversed with exogenous MMP‐3 stimulation ( P < 0.05). Conclusions Interleukin‐1 β induces MMP‐3‐regulated cell proliferation and suppresses apoptosis in dental pulp cells.