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Microbial composition of atherosclerotic plaques
Author(s) -
Calandrini CA,
Ribeiro AC,
Gonnelli AC,
OtaTsuzuki C,
Rangel LP,
SabaChujfi E,
Mayer MPA
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12205
Subject(s) - phylotype , periodontitis , microbiology and biotechnology , aorta , endarterectomy , pathology , biology , chronic periodontitis , carotid endarterectomy , myocardial infarction , medicine , bacteria , 16s ribosomal rna , carotid arteries , genetics
Objective The association of infections such as periodontitis with atherosclerotic diseases is well documented. In spite of the high diversity of the human oral microbiota, and its close contact with the circulatory system, few oral species were detected in atherosclerotic plaques. Thus, we attempted to evaluate the microbial diversity of atherosclerotic plaques from patients with different periodontal conditions, submitted to endarterectomy by a broad‐range microbial method. Materials and Methods Patients indicated for aorta endarterectomy due to myocardial infarction were recruited for periodontal clinical examination. The microbial diversity of atherosclerotic plaques ( n = 35) was evaluated by sequence analysis of bacterial 16 S r RNA libraries. Results Bacterial DNA was detected in 12 endarterectomy specimens (34.3%). Twenty‐three bacterial species/phylotypes were identified. P roteobacteria and F irmicutes comprised 78.3% and 21.7% of the identified taxa, respectively. Fifteen (60.9%) phylotypes were reported as yet uncultivable or as yet uncharacterized species. Two uncultured phylotypes were previously detected in the human mouth. The periodontopathogen A ggregatibacter actinomycetemcomitans was detected in seven samples (20%), followed by P seudomonas species. There was no association between periodontal parameters and detection of A . actinomycetemcomitans or other phylotypes in atherosclerotic plaques. Conclusion Our results suggest a role of the oral microbiota in the development of inflammation in atherogenesis, particularly of A . actinomycetemcomitans .