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Concentration of salivary protective proteins within the bound oral mucosal pellicle
Author(s) -
Gibbins HL,
Proctor GB,
Yakubov GE,
Wilson S,
Carpenter GH
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12194
Subject(s) - mucin , saliva , secretory component , chemistry , glycoprotein , immune system , secretory protein , secretory iga , blot , immunoglobulin a , salivary gland , immunology , microbiology and biotechnology , biology , antibody , biochemistry , secretion , immunoglobulin g , gene
Objectives To study which salivary proteins form the protective bound mucosal pellicle and to determine the role of transglutaminase in pellicle development. Materials and Methods Oral epithelial cells were collected and underwent washes of different strengths, followed by homogenisation. SDS ‐ PAGE , western blotting, IgA ELISA s and amylase activity assays were completed on cell homogenates and compared to saliva samples to confirm which salivary proteins were bound to cell surfaces. Results Salivary mucins, MUC 5B and MUC 7, were strongly retained on the oral epithelial cell surface. Other bound proteins including cystatin S, carbonic anhydrase VI , secretory component and IgA could be washed off. IgA was present in concentrated levels in the bound mucosal pellicle compared to amounts in saliva. Amylase, one of the most abundant proteins present in saliva, showed minimal levels of binding. Transglutaminase 3 presence was confirmed, but proteins that it catalyses cross‐links between, statherin and proline‐rich proteins, showed minimal presence. Conclusion Some protective salivary proteins including mucins and IgA become concentrated on oral surfaces in the bound mucosal pellicle, through specific interactions. Concentration of mucins would contribute to lubrication to prevent abrasion damage to soft tissues, whilst increased IgA could create an ‘immune reservoir’ against mucosal infection.