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Association of MMP but not TIMP ‐1 gene polymorphisms with recurrent aphthous stomatitis
Author(s) -
Karasneh JA,
BaniHani ME,
Alkhateeb AM,
Hassan AF,
Thornhill MH
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12190
Subject(s) - recurrent aphthous stomatitis , haplotype , genotype , linkage disequilibrium , immunology , medicine , biology , genetics , gastroenterology , stomatitis , gene
Objectives Recurrent aphthous stomatitis ( RAS ) is a common oral inflammatory disease induced by genetic and environmental factors. Gelatinases ( MMP ‐2 and MMP ‐9) and their natural inhibitor TIMP ‐1 are active players in the inflammatory process. We aimed to determine whether inheritance of specific MMP ‐2, MMP ‐9, or TIMP ‐1 gene polymorphisms is associated with RAS susceptibility. Subjects and methods Ninety‐six RAS patients and 153 healthy controls were studied. Five polymorphisms were genotyped: rs17576, rs3918242, and rs11697325 in MMP ‐9, MMP ‐2 rs2285053, and TIMP ‐1 rs6609533. Association was assessed by logistic regression analysis after adjustment for confounding factors. Linkage disequilibrium ( LD ) was assessed using the Haploview program. Results MMP ‐9 rs11697325 was significantly associated with RAS , with an increase in the AA genotype in patients, determined using χ 2 analysis ( OR = 2.3, P = 0.006) and adjusted regression analysis ( OR = 3.1, P = 0.009). MMP ‐9 rs11697325 and rs17576 showed strong LD ( D ' = 0.95), with an increase in the AA haplotype ( P = 0.023) and a decrease in the GA haplotype ( P = 0.015) in patients. Conclusions This is the first study to investigate the association of MMP s or TIMP ‐1 with RAS . We found a significant association between MMP ‐9 rs11697325 polymorphisms and RAS . Confirmatory studies in other populations and functional investigations are needed to determine the role of these genes in RAS .