Premium
Human periodontal ligament cell sheets cultured on amniotic membrane substrate
Author(s) -
Adachi K,
Amemiya T,
Nakamura T,
Honjyo K,
Kumamoto S,
Yamamoto T,
Bentley AJ,
Fullwood NJ,
Kinoshita S,
Kanamura N
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12176
Subject(s) - periodontal fiber , adhesion , microbiology and biotechnology , regeneration (biology) , cell culture , cell , amnion , cell adhesion , chemistry , membrane , immunofluorescence , anatomy , biology , immunology , medicine , dentistry , fetus , antibody , pregnancy , biochemistry , genetics , organic chemistry
Objective Periodontal ligament ( PDL ) cells and their substrates play key roles in periodontal regeneration. However, there has been no report on the use of amniotic membrane ( AM ) as a substrate for culturing PDL cells. In the current study, we conducted an analysis of PDL cells cultivated on AM to determine the distribution of factors responsible for maintaining the characteristics of PDL . Materials and Methods Amniotic membrane was obtained from women undergoing cesarean sections, whereas PDL tissue was obtained from human maxillary third molars. The harvested PDL cells were maintained in explant culture for three or four passages, following which they were cultured on AM . Results After 3 weeks of culture, the PDL cells had grown well on AM . Immunofluorescence showed that these cells were capable of proliferating and potentially maintaining their PDL ‐like properties. In addition, strong cell–cell adhesion structures, namely desmosomes and tight junctions, were shown to be present between cells. Electron microscopy images showed that the cultured PDL cells had differentiated and proliferated on AM with lateral conjugation and adhesion to AM . Conclusion We conclude that AM may represent a suitable substrate for culturing PDL cells and that PDL cells cultured on AM show sheet formation.