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Mitochondrial polymorphisms and dysfunction related to aggressive periodontitis: a pilot study
Author(s) -
Wang X,
Luan Q,
Chen Q,
Zhao L,
Guo Y
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12163
Subject(s) - mitochondrial dna , reactive oxygen species , periodontitis , aggressive periodontitis , biology , medicine , mitochondrion , snp , genotype , point mutation , genetics , single nucleotide polymorphism , gene , mutation
Aim To investigate whether aggressive periodontitis ( A gP) is associated with specific mt DNA polymorphisms or point mutations and furthermore whether mitochondrial dysfunction occurs in gingival fibroblasts of A gP patients. Materials and methods The mitochondrial DNA coding regions were amplified and sequenced in 22 overlapped fragments in 34 patients with A gP and 28 healthy controls for initial screening. We selected eleven SNP s for detailed investigation in the rest 30 A gP patients and 26 healthy controls, because all other variants occurred at relatively low frequencies or had no difference between two groups. Logistic regression models were used to analyze the association between mt DNA variants and A gP. Gingival fibroblasts were cultured from four patients with A gP and four healthy controls, and then mitochondrial membrane potential, reactive oxygen species production and cell proliferation were analyzed. Results Significant association was observed between aggressive periodontitis and eight mitochondrial polymorphisms: ‘8701A‐9540T‐10400C‐10873T‐14783T‐15043G‐15301G’ ( OR = 3.471 (1.610–7.483), P = 0.001) and 10398A ( OR = 3.238 (1.481–7.078), P = 0.003). Compared with the controls, patients with aggressive periodontitis had a decrease in mitochondrial membrane potential and an increase in reactive oxygen species production. Conclusion In conclusion, we propose that mitochondrial dysfunction and ‘8701A‐9540T‐10400C‐10873T‐14783T‐15043G‐15301G, 10398A’ are associated with and may increase the susceptibility to A gP in the Han Chinese population.