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Association of TLR2, TLR3, TLR4 and CD14 genes polymorphisms with oral cancer risk and survival
Author(s) -
Zeljic K,
Supic G,
Jovic N,
Kozomara R,
BrankovicMagic M,
Obrenovic M,
Magic Z
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12144
Subject(s) - genotype , haplotype , medicine , linkage disequilibrium , biology , oncology , gene , genetics
Objectives The aim of this study is to investigate association between polymorphisms in TLR 2, TLR 3, TLR 4 and CD 14 genes or their haplotypes with oral squamous cell carcinomas ( OSCC ) risk and survival. Methods The study was conducted on 93 OSCC patients and 104 cancer‐free controls. Polymorphisms were genotyped by real‐time PCR or PCR‐RFLP method. Results Significant increase in oral cancer risk was observed in individuals with mutated genotype of TLR 3 rs3775291 polymorphism (OR = 1.096, P = 0.036) compared to wild‐type . The heterozygous and mutated genotype of TLR 3 rs5743312 polymorphism had worse survival in group of patients with stage III tumours ( P = 0.043). Multivariate Cox regression analysis revealed that TLR 3 rs5743312 polymorphism could be considered as prognostic marker in advanced III stage OSCC (HR = 2.456, P = 0.007), but not independently of nodal status. TLR3 rs3775291 and rs5743312 polymorphisms were in strong linkage disequilibrium. Haplotype TG was associated with worse prognosis in OSCC patients in comparison with common CG haplotype (HR = 1.717, P = 0.042). Interaction among polymorphisms in TLR2, TLR3 and CD14 genes was observed ( P = 0.010). Conclusions TLR3 rs5743312 polymorphism could be considered as potential predictor of worse overall survival in advanced oral cancer, but not independently of nodal status. Haplotypes in TLR3 gene might be associated with poor prognosis in OSCC patients.