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Dysregulation of mi R ‐31 and mi R ‐375 expression is associated with clinical outcomes in oral carcinoma
Author(s) -
Siow MY,
Karen Ng LP,
Vincent Chong VK,
Jamaludin M,
Abraham MT,
Abdul Rahman ZA,
Kallarakkal TG,
Yang YH,
Cheong SC,
Zain RB
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12118
Subject(s) - pathological , rna , microrna , microarray , carcinoma , immunohistochemistry , pathology , medicine , univariate analysis , cancer research , oncology , gene expression , biology , multivariate analysis , gene , biochemistry
Objectives To identify differentially expressed mi RNA between oral squamous cell carcinoma ( OSCC ) and non‐cancer ( NC ) and to associate these with clinico‐pathological parameters. Materials and methods mi RNA microarray profiling was utilized to obtain the expression profile of mi RNA s in four OSCC and four NC samples. The expression of miR‐31 and miR‐375 was further validated in 26 OSCC and three NC samples using real‐time‐ PCR . The association between mi RNA expression and clinico‐pathological parameters was tested by univariate and multivariate analyses. Results Microarray profiling demonstrated that 15 and four mi RNA s were up‐regulated and down‐regulated, respectively, in OSCC as compared with NC . miR‐31 and miR‐375 were validated as up‐ and down‐regulated mi RNA s, respectively. In univariate analyses, expression of miR‐31 was significantly elevated in early stage, tumours with no metastatic nodes and those from the buccal mucosa. By contrast, low miR‐375 expression was significantly associated with late stage disease, larger tumour size and the non‐cohesive type of pattern of invasion in OSCC . The association between miR‐31 expression with tumour staging and site and miR‐375 with tumour staging remained significant in multivariate analyses. Conclusions This study has identified 19 mi RNA s significantly associated with OSCC , and expressions of miR‐31 and miR‐375 were significantly related with clinico‐pathological parameters suggesting they could be important in driving oral tumourigenesis.