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Mandibular symphysis and ramus as sources of osteoblastic cells for bone tissue engineering
Author(s) -
Ferraz EP,
Xavier SP,
Oliveira PT,
Beloti MM,
Rosa AL
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12115
Subject(s) - bone sialoprotein , runx2 , osteocalcin , osteopontin , osteoblast , alkaline phosphatase , extracellular matrix , homeobox , mandibular symphysis , chemistry , biology , microbiology and biotechnology , symphysis , gene expression , anatomy , endocrinology , gene , biochemistry , in vitro , enzyme
Objectives Autografts from mandibular symphysis and ramus are often used for bone reconstruction. Based on this, we hypothesized that these sites could be useful cell sources for bone tissue engineering approaches. Thus, our study aimed at evaluating the proliferation and osteoblast phenotype development of cells derived from mandibular symphysis and ramus. Materials and Methods Cells were isolated from bone fragments of four patients by enzymatic digestion and cultured under osteogenic condition for up to 17 days. Cultures were assayed for cell proliferation, gene expression of key bone markers runt‐related transcription factor 2 (Runx2), distal‐less homeobox 5 ( DLX 5), SATB homeobox 2 ( SATB 2), Osterix ( OSX ), family with sequence similarity 20, member C ( FAM 20C), bone sialoprotein ( BSP ), osteopontin ( OPN ) and osteocalcin ( OC ), alkaline phosphatase ( ALP ) expression and activity, and extracellular matrix mineralization. Data were compared by two‐way ANOVA or t ‐test for independent samples when appropriate. Results Cells derived from ramus displayed lower proliferative activity and higher gene expression of Runx2, DLX 5, SATB 2, OSX , FAM 20C, BSP , OPN and OC , ALP protein expression and activity and extracellular matrix mineralization compared with symphysis‐derived cells. Conclusion Symphysis and ramus may be considered as cell sources for bone tissue engineering approaches but due to the higher osteogenic potential, ramus‐derived cells are more appealing for constructing cell‐based biomaterials.