Premium
The association of IL ‐6 and IL ‐6R gene polymorphisms with chronic periodontitis in a Chinese population
Author(s) -
Zhang HY,
Feng L,
Wu H,
Xie XD
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12075
Subject(s) - chronic periodontitis , single nucleotide polymorphism , haplotype , periodontitis , genotype , odds ratio , aggressive periodontitis , interleukin , immunology , medicine , biology , genetics , gene , cytokine
Objective The aim of our investigation is to reveal the association of interleukin‐6 ( IL ‐6 ) and interleukin‐6 receptor ( IL ‐6R ) gene polymorphisms with chronic periodontitis in Northwestern Chinese Han Population. Material and Methods A case–control study of 199 cases of chronic periodontitis patients and 216 healthy controls was performed. Genomic DNA was isolated from whole venous blood, and four single nucleotide polymorphisms ( IL ‐6 −572 G/C and −1363 G/T, IL ‐6R −183 G/A and +48892 A/C) were analysed using polymerase chain reaction, followed by restriction fragment length polymorphism analysis and sequencing methods. Results Our SNP analyses showed that the distribution of the IL ‐6 −572 G/C and IL ‐6R −183 G/A polymorphisms did not differ between patients and controls. The frequency of the IL ‐6 −1363 G/T genotype GG was significantly increased chronic periodontitis cases ( P = 0.023, odds ratio ( OR ) = 2.825 adjusted for gender and age.) The IL ‐6R +48892 A/C polymorphisms genotype CC was found to be protective against chronic periodontitis ( P = 0.004, OR = 0.318 adjusted for gender and age.). In addition, compared with the other haplotypes, haplotype A +48892 A −183 was significantly associated with chronic periodontitis, with an odds ratio of 0.720 ( P = 0.0235). Conclusion Our results suggest that the IL ‐6 −1363 G/T and IL ‐6R +48892 A/C polymorphisms may contribute to genetic susceptibility to chronic periodontitis in Northwestern Chinese Han population.