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Serum micro RNA s as potential biomarkers of mandibular prognathism
Author(s) -
Gu Y,
Zhang Y,
Zhao C,
Pan Y,
Smales RJ,
Wang H,
Ni Y,
Zhang H,
Ni J,
Ma J,
Wang L
Publication year - 2014
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12073
Subject(s) - mandibular prognathism , medicine , receiver operating characteristic , dentition , microrna , stage (stratigraphy) , dentistry , molar , orthodontics , biology , osteotomy , gene , genetics , paleontology
Objectives The aim of the study was to determine whether the expression levels of specific circulating serum micro RNA s (mi RNA s) are associated with mandibular prognathism ( MP ). Methods Sixty subjects in the early permanent dentition stage and 23 in the mixed dentition stage with MP were identified. Sixty‐eight normal control subjects in the early permanent dentition stage and 24 in the mixed dentition stage were recruited for comparison. According to the microarray‐based expression profiling, four serum mi RNA s (let‐7i‐3p, mi R ‐595, mi R ‐16‐2‐3p, and mi R ‐367‐5p) were validated. Results In the MP groups, let‐7i‐3p was significantly over‐expressed in subjects in the early permanent ( P < 0.0005) and mixed ( P < 0.001) dentitions, and mi R ‐595 was significantly under‐expressed ( P < 0.004) in subjects in the early permanent ( P < 0.004) and mixed ( P < 0.0005) dentitions, compared with normal control groups. Multiple logistic regression analysis and receiver operating characteristic curve analysis revealed that let‐7i‐3p and mi R ‐595 were able to significantly discriminate MP subjects from normal controls. Conclusion Let‐7i‐3p and mi R ‐595 could be potential, non‐invasive biomarkers for the accurate early detection and diagnosis of MP , which may result in improved clinical management.