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Effects of tobacco on cytokine expression from human endothelial cells
Author(s) -
Allam E,
DelaCruz K,
Ghoneima A,
Sun J,
Windsor LJ
Publication year - 2013
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12050
Subject(s) - cytokine , nicotine , immunology , pathogenesis , medicine , chemistry , biology , pharmacology
Objective To investigate the effects of nicotine and cigarette smoke condensate ( CSC ) exposure on cytokine expression from human endothelial cells in order to identify one possible mechanism that smoking plays in the pathogenesis of both periodontal disease ( PDD ) and cardiovascular disease ( CVD ). Methods Human endothelial cells ( HUVEC s) were exposed to different concentrations of nicotine and CSC to examine the effects that they have on cell proliferation and cytotoxicity. Non‐toxic levels were then used to examine cytokine expression using cytokine protein arrays. Results Exposure to nicotine caused significant down‐regulation in the expression of IL ‐10 ( P = 0.046), growth‐regulated oncogene ( GRO )α ( P = 0.036), MCP ‐1 ( P = 0.046), and GMCSF ( P = 0.004) compared with the control untreated HUVEC s. Exposure to CSC caused significant down‐regulation in the expression of GRO ( P = 0.04), GRO α ( P = 0.01), IL ‐6 ( P = 0.03), and MCP ‐1 ( P = 0.04) compared with the control untreated HUVEC s. Conclusions Exposure of HUVEC s to nicotine or CSC affects the levels of cytokine expression including reduction in anti‐inflammatory and chemoattractant cytokines. This may subsequently affect the host defensive mechanisms of the tissues. The action of toxic chemicals in tobacco smoke on endothelial cells is a potential pathogenic mechanism that may in part explain the association between tobacco, PDD , and CVD .