Premium
Association of CD 28 and CTLA ‐4 gene polymorphisms with aggressive periodontitis in Brazilians
Author(s) -
e Silva MRMA,
Moreira PR,
da Costa GC,
Saraiva AM,
Souza PEA de,
Amormino SAF,
Costa JE da,
Gollob KJ,
Dutra WO
Publication year - 2013
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12036
Subject(s) - aggressive periodontitis , periodontitis , genotype , genotyping , chronic periodontitis , immunology , genetic predisposition , polymorphism (computer science) , gene , genetics , biology , medicine
Objective Susceptibility to and severity of periodontal disease is influenced by gene polymorphisms related to the immune response. Co‐stimulatory molecules, such as CD 28 and CTLA ‐4, are critical in the development of such responses. Our hypothesis is that polymorphisms in genes that code for these molecules may be associated with periodontitis. The aim of the study was to investigate the association between +17 (T/C) CD 28 and +49 (A/G) CTLA ‐4 gene polymorphisms and periodontitis in Brazilians. Materials and methods Genomic DNA was obtained from oral swabs of 424 individuals categorized into three groups (control group, aggressive, and chronic periodontitis) considering clinical parameters such as probing depth and clinical attachment loss. The genotyping was performed by polymerase chain reaction–restriction fragment length polymorphism. Results There was an association between the T − genotype of the CD 28 polymorphism and aggressive periodontitis ( P = 0.04). Moreover, the A + genotype for CTLA ‐4 was associated with greater clinical attachment loss in non‐smokers with aggressive periodontitis ( P = 0.006, OR = 16.25, CI = 2.25–117.11). Conclusions These findings show that T − in CD 28 + 17 (T/C) and the A + in CTLA ‐4 +49 (A/G) genotypes are associated with susceptibility to aggressive periodontal disease. Thus, our study highlights these polymorphisms as potential genetic susceptibility markers of periodontitis in Brazilians.