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The in vitro study of ursolic acid and oleanolic acid inhibiting cariogenic microorganisms as well as biofilm
Author(s) -
Zhou L,
Ding Y,
Chen W,
Zhang P,
Chen Y,
Lv X
Publication year - 2013
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12031
Subject(s) - biofilm , ursolic acid , oleanolic acid , streptococcus mutans , chemistry , microbiology and biotechnology , antibacterial activity , crystal violet , microorganism , minimum inhibitory concentration , bacteria , minimum bactericidal concentration , antimicrobial , biology , chromatography , medicine , genetics , alternative medicine , pathology
Objective The aim of this study was to examine the effect of ursolic acid ( UA ) and oleanolic acid ( OA ), triterpenoid compounds that are isolated from many edible and medicinal plants, on cariogenic microorganisms and biofilms. Methods A microtitre plate dilution assay was used to determine the minimum inhibitory concentration ( MIC ) and minimum bactericidal concentration ( MBC ) of UA and OA against two A ctinomyces spp. and four S treptococcus spp. The antibacterial activity of UA and OA was assessed by crystal violet staining, scanning electron microscopy ( SEM ) and confocal laser scanning microscopy ( CLSM ). Results UA and OA displayed differential antibacterial activities against the six tested bacteria, with UA showing greater antibacterial activity than OA . Furthermore, the two drugs had greater antibacterial activity against A ctinomyces spp. than S treptococcus spp. UA and OA at 1/4 MIC can reduce bacterial biofilm formation, whereas higher UA concentrations displayed antibacterial activity against Actinomyces viscosus and Streptococcus mutans in mature biofilms. For instance, 2.0 mg ml −1 UA was sufficient to kill an A. viscosus biofilm. Conclusions UA and OA inhibit the growth of cariogenic microorganisms, which suggests that UA and OA have considerable potential as antibacterial agents for dental caries prevention.