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Expression of proliferative markers in ameloblastomas and malignant odontogenic tumors
Author(s) -
Otero D,
Lourenço SQC,
RuizÁvila I,
Bravo M,
Sousa T,
Faria PAS,
GonzálezMoles MA
Publication year - 2013
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/odi.12010
Subject(s) - ameloblastoma , immunohistochemistry , immunostaining , pathology , odontogenic tumor , pathological , medicine , biology , odontogenic , molar , dentistry
Objective To compare the proliferative activity in ameloblastoma and malignant odontogenic tumors, as assessed by K i‐67 immunostaining and determine whether expression of substance P ( SP ) and NK ‐1 receptor ( NK ‐1 R ) is related to cell proliferation in these tumors. Materials and methods Immunohistochemistry was used to evaluate protein expression in 44 benign and malignant odontogenic tumors from 39 patients. Immunohistochemistry was performed with anti‐ SP , anti‐ NK ‐1 R , and anti‐ K i‐67 monoclonal antibodies, and the clinical and pathological data of the patients with odontogenic tumor were evaluated. Results Expression of K i‐67 in malignant odontogenic tumors was significantly higher than in ameloblastomas ( P < 0.001), and the expression level was associated with higher expression of NK ‐1 R . Among the ameloblastomas, there was significantly higher expression of K i‐67 in peripheral ameloblastic‐like cells (3.3 ± 4.1) than in stellate reticulum‐like cells (2.6 ± 3.7) ( P = 0.04). In the majority of tissue locations of the malignant tumors, expression of SP and NK ‐1 R was positively correlated with higher expression of K i‐67. Conclusion These findings show that the expression level of K i‐67 in ameloblastomas was positively correlated with the rate of growth of odontogenic tumors. Overexpression of NK ‐1 R complex in malignant odontogenic tumors could be part of the trigger stimulus that results in higher proliferative activity of the tumor.