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Enhancement of post‐orthodontic tooth stability by carbonated hydroxyapatite‐incorporated advanced platelet‐rich fibrin in rabbits
Author(s) -
Alhasyimi A. A.,
Pudyani P. P.,
Asmara W.,
Ana I. D.
Publication year - 2018
Publication title -
orthodontics and craniofacial research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 55
eISSN - 1601-6343
pISSN - 1601-6335
DOI - 10.1111/ocr.12224
Subject(s) - platelet rich fibrin , fibrin , osteoclast , dentistry , tartrate resistant acid phosphatase , haematoxylin , chemistry , osteoblast , medicine , staining , pathology , biochemistry , in vitro , receptor , immunology
Structured Abstract Objective To evaluate the effect of carbonated hydroxyapatite‐incorporated advanced platelet‐rich fibrin on relapse and bone remodelling in rabbits. Materials and Methods Forty‐five rabbits were divided into 3 groups (n = 15): a control group, carbonated hydroxyapatite ( CHA ) and carbonated hydroxyapatite‐incorporated advanced platelet‐rich fibrin ( CHA ‐ aPRF ) group. The lower incisors were subjected to an orthodontic force of 50 cN . During the retention period, CHA and CHA ‐ aPRF hydrogel were gently injected in the mesial gingival sulcus every 7 days. Next, the appliances were debonded to allow relapse. At 5 subsequent time points (0, 3, 7, 14 and 21 days after debonding), the distance between the mesial tips of rabbits incisors was measured using a digital calliper, osteoblast quantity was evaluated using haematoxylin‐eosin staining and osteoclast activity was examined using tartrate‐resistant acid phosphatase ( TRAP ) staining. Results The relapse rate of CHA ‐ aPRF group was lower than that of the other groups, and the relapse distances were significantly lower on days 14 and 21 ( P < .05). Histological analysis revealed that the number of osteoblasts was significantly higher and that the osteoclast activity was significantly lower in the CHA ‐ aPRF group compared with the other groups ( P < .05). Conclusions Intrasulcular injection of hydrogel CHA incorporated aPRF can locally reduce orthodontic relapse in rabbits.