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Appropriate cyclic tensile strain promotes biological changes of cranial base synchondrosis chondrocytes
Author(s) -
Chu F.,
Feng Q.,
Hu Z.,
Shen G.
Publication year - 2017
Publication title -
orthodontics and craniofacial research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 55
eISSN - 1601-6343
pISSN - 1601-6335
DOI - 10.1111/ocr.12194
Subject(s) - synchondrosis , sox9 , chondrocyte , extracellular matrix , cartilage , andrology , immunohistochemistry , endocrinology , medicine , anatomy , chemistry , microbiology and biotechnology , gene expression , biology , biochemistry , gene
Structured Abstract Objectives This study was designed to clarify biological changes of cranial base synchondrosis chondrocytes ( CBSC s) upon cyclic tensile strain ( CTS ) loading which simulated orthopaedic mechanical protraction on cranial base synchondroses ( CBS ). Material and Methods A two‐step digestion method was used to isolate CBSC s obtained from 1‐week‐old Sprague Dawley rats. Immunohistochemical staining of type II collagen and Sox9 was conducted to identify chondrocytes. A CTS of 1 Hz and 10% elongation was applied to the second passage of CBSC s by FX ‐5000™ Tension System for 24 hours. The control group kept static at the same time. The expression levels of extracellular matrix ( Acan , Col1a1 , Col2a1 and Col10a1 ) and key regulatory factors ( Sox9 , Ihh and PTH rP ) were detected by quantitative real‐time RT ‐ PCR . Results Positive staining of type II collagen and Sox9 was detected in the isolated CBSC s. The relative expression level of Acan , Col2a1 , Col10a1 , Sox9 and Ihh in the CTS ‐loading group was 1.85‐fold, 2.19‐fold, 1.53‐fold, 6.62‐fold, and 1.39‐fold, respectively, as much as that in the control group, which had statistical significance ( P <.05). There was no statistical difference ( P >.05) in the expression of Col1a1 and PTH rP . Conclusions A CTS of 1 Hz and 10% elongation for 24 hours had positive effects on chondrocyte proliferation, phenotype maintenance and cartilage matrix synthesis.