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Osteoprotegerin and zoledronate bone effects during orthodontic tooth movement
Author(s) -
FernándezGonzález F. J.,
LópezCaballo J. L.,
Cañigral A.,
MenéndezDíaz I.,
Brizuela A.,
Cos F. J.,
Cobo T.,
Vega J. A.
Publication year - 2016
Publication title -
orthodontics and craniofacial research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 55
eISSN - 1601-6343
pISSN - 1601-6335
DOI - 10.1111/ocr.12115
Subject(s) - osteoprotegerin , molar , medicine , periodontal fiber , matrix metalloproteinase , rankl , bisphosphonate , bone resorption , dentistry , bone remodeling , chemistry , orthodontics , receptor , osteoporosis , activator (genetics)
Structured Abstract Objectives To assess the effects of local delivery of recombinant fusion protein osteoprotegerin ( OPG ‐Fc) and bisphosphonate zoledronate on bone and periodontal ligament in a rat tooth movement model. Materials and methods Maxillary first molars of 36 male Sprague–Dawley rats were displaced mesially using a calibrated spring connected to an anterior mini‐screw. Two different drugs were used: a single dose of Zoledronate (16 μg) and a twice‐weekly dose of OPG ‐Fc (5.0 mg/kg) were injected. Tooth movement was measured on scanned plaster casts. Structural and immunohistochemical analysis of the orthodontic‐induced changes in bone included receptor activator of nuclear factor ĸ ( RANK ), Runx, type 1 collagen, matrix metalloproteinases (MMPs) 2 and 9, tissue inhibitors of metalloproteinases (TIMPs) 1 and 2, and vimentin. Results Both groups showed a reduction in mesial molar displacement. Animals receiving OPG ‐Fc demonstrated only 52%, 31%, and 21% of the total mesial molar displacement compared to control rats at 7, 14, and 21 days, respectively (* p < 0.001). For rats receiving zoledronate tooth displacement decreased significantly with 52%, 46% and 30%, respectively (* p < 0.001). At 14 and 21 days, OPG ‐Fc group showed significantly less molar displacement than the zoledronate group (* p < 0.001). RANK , Runx, vimentin, MMP‐9 and tissues‐inhibitor metalloproteinase 1 immunoreactivity were reduced in zoledronate treated animals and even more in OPG treated animals. Conclusion Local delivery of OPG ‐Fc or zoledronate inhibits bone resorption and therefore tooth movement. OPG ‐Fc was more effective than zoledronate in blocking the action of osteoclasts.