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Evaluation of BSP expression and apoptosis in the periodontal ligament during orthodontic relapse: a preliminary study
Author(s) -
McManus A.,
Utreja A.,
Chen J.,
Kalajzic Z.,
Yang W.,
Nanda R.,
Wadhwa S.,
Uribe F.
Publication year - 2014
Publication title -
orthodontics and craniofacial research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 55
eISSN - 1601-6343
pISSN - 1601-6335
DOI - 10.1111/ocr.12049
Subject(s) - periodontal fiber , apoptosis , molar , chemistry , bone sialoprotein , dental alveolus , osteoclast , dentistry , medicine , in vitro , biochemistry , alkaline phosphatase , osteocalcin , enzyme
Structured Abstract Objective To examine the expression of bone sialoprotein ( BSP ) and apoptosis in an in vivo orthodontic relapse model. Materials and Methods Male mice (10–12 weeks old), either transgenic [green fluorescent protein ( GFP ) driven by the BSP promoter] or wild type, were used in this study. To achieve orthodontic tooth movement ( OTM ), maxillary right first molars were moved mesially using closed‐coil springs. Animals were divided into an OTM group (14 days continuous orthodontic force – 11 animals) or Relapse group (10 days of force application followed by 4 days of relapse – 8 animals). The control group was comprised of the contralateral maxillary molars. The periodontal ligament ( PDL ) was analyzed in areas of compression and tension for transgenic expression, osteoclast localization, and the presence of apoptotic cells. Results There was a significant decrease in GFP ‐labeled cells on the compression and tension sides of the PDL in the OTM group compared with control. In the relapse group, GFP ‐labeled cells were significantly decreased only on the old compression side. Osteoclasts were localized on the compression side of the OTM group, whereas in the R elapse group, they were present on both sides. PDL apoptosis significantly increased on the compression side in OTM and R elapse groups. Conclusion Both OTM and R elapse groups exhibited a decreased number of GFP ‐labeled cells in areas of compression and tension. There was significant PDL apoptosis in regions under compressive forces following OTM and to a lesser extent following relapse.