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The effect of IL ‐17 on the production of proinflammatory cytokines and matrix metalloproteinase‐1 by human periodontal ligament fibroblasts
Author(s) -
Shibata M.,
Shintaku Y.,
Matsuzaki K.,
Uematsu S.
Publication year - 2014
Publication title -
orthodontics and craniofacial research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 55
eISSN - 1601-6343
pISSN - 1601-6335
DOI - 10.1111/ocr.12033
Subject(s) - proinflammatory cytokine , periodontal fiber , matrix metalloproteinase , microbiology and biotechnology , interleukin , interleukin 1β , matrix (chemical analysis) , metalloproteinase , matrix metalloproteinase 9 , matrix metalloproteinase 3 , chemistry , inflammation , immunology , medicine , cytokine , biology , dentistry , biochemistry , chromatography
Structured Abstract Objectives To investigate the effects of IL ‐17 on IL ‐6, IL ‐1β, and matrix metalloproteinase ( MMP ‐1) production, and to compare the MMP ‐1 production between the individual and combined effects of IL ‐1β and IL ‐6 in human periodontal ligament fibroblasts ( HPDLF ). Materials and Methods Human periodontal ligament fibroblasts were cultured with IL‐17 for 0.5, 1, 4, 24, 48, and 72 h, and were cultured with IL‐1β, IL‐6/s IL ‐6R, or a combination of IL‐1β and IL‐6/s IL ‐6R for 24 h. To measure the m RNA levels of IL‐6 , IL‐1β, and MMP‐1 , total RNA was extracted from the cultured HPDLF, and a real‐time PCR analysis was performed. The protein levels of IL‐6, IL‐1β, and MMP‐1 in supernatants were measured using enzyme‐linked immunosorbent assays (ELISAs). Results IL‐17 significantly increased the expression of IL‐6 and MMP‐1 m RNA and protein, while IL‐17 transiently increased the expression of IL‐1β m RNA . The combination of IL‐1β and IL‐6/ sIL ‐6R induced significantly higher levels of MMP‐1 protein than IL‐1β alone. Conclusions IL‐17 upregulated the production of IL‐6 and MMP‐1 sequentially in HPDLF. IL‐6/s IL ‐6R may enhance the effects of IL‐1β on MMP‐1 production. The present results suggest that IL‐17 induces MMP‐1 production not only directly, but also indirectly by promoting IL‐6 production, thus resulting in the degradation of collagens in the PDL.