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Comparison of methodological approaches to human gut microbiota changes in response to metabolic and bariatric surgery: A systematic review
Author(s) -
MoralesMarroquin Elisa,
Hanson Blake,
Greathouse Leigh,
CruzMunoz Nestor,
Messiah Sarah E.
Publication year - 2020
Publication title -
obesity reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.845
H-Index - 162
eISSN - 1467-789X
pISSN - 1467-7881
DOI - 10.1111/obr.13025
Subject(s) - akkermansia muciniphila , sleeve gastrectomy , gut flora , firmicutes , human gastrointestinal tract , biology , microbiome , medicine , bioinformatics , obesity , gastric bypass , weight loss , gastrointestinal tract , bacteria , immunology , genetics , 16s ribosomal rna
Summary Substantial differences in the response of gut microbial composition to metabolic and bariatric surgery have been reported. Therefore, the goal of the present review is to evaluate if methodological differences could be driving this lack of consistency. A search was conducted using PUBMED, Web of Science, Science Direct and COCHRANE using the following inclusion criteria: human studies written in English with a baseline sampling point, using gut microbiota as an outcome and either Roux‐n‐Y gastric bypass or sleeve gastrectomy. Sixteen articles were selected (total 221 participants). Roux‐n‐Y gastric bypass caused more alterations in gut microbial composition in comparison with sleeve gastrectomy. Substantial variability was found in study designs, data collection and analyses across studies. Increases in several families and genera from the phylum Proteobacteria and Bacteroidetes, the family Streptococcaceae, the species Akkermansia muciniphila and Streptococcus salivarius and a decrease in the phylum Firmicutes and the family Bifidobacteriaceae were reported. There is a need for standardization not only of microbial analysis but also of study designs when analysing the effect of bariatric surgery on the human gut microbiome. In addition, outcomes from different surgical procedures should not be combined as they produce distinctive effects on gut microbial composition.