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Comparative proteomic analysis reveals novel potential virulence factors of Aeromonas veronii
Author(s) -
Yang BinTong,
Sun YuFeng,
Cao LiNan,
Raza Sayed Haidar Abbas,
Zhou JinHua,
Li YaNan,
Sun WuWen,
Wang GuiQin,
Shan XiaoFeng,
Kang YuanHuan,
Qian AiDong
Publication year - 2021
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/nyas.14480
Subject(s) - virulence , aeromonas veronii , aerolysin , biology , kegg , microbiology and biotechnology , proteome , pathogen , pathogenesis , gene , genetics , aeromonas , gene expression , bacteria , transcriptome , immunology
Aeromonas veronii is an important zoonotic and aquatic pathogen. An increasing number of reports indicate that it has caused substantial economic losses in the aquaculture industry, in addition to threatening human health. However, little is known about its pathogenesis. Exploration of new virulence factors of A. veronii would be helpful for further understanding its pathogenesis. Hence, we comparatively analyzed the proteomes of virulent, attenuated, and avirulent strains of A. veronii using tandem mass tag (TMT) protein labeling and found numerous proteins either up‐ or downregulated in the virulent strain. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that these differentially expressed proteins (DEPs) were involved mainly in pathways associated with bacterial chemotaxis and microbial metabolism in diverse environments. Furthermore, the expression levels of lysine decarboxylase, endoribonuclease, maltoporin, pullulanase, and aerolysin were positively correlated with the virulence of the strains, suggesting that their function may be closely related to the virulence of A. veronii . The results of qRT‐PCR and multiple reaction monitoring for some DEPs were consistent with the results of TMT protein labeling. These results suggest that these DEPs may be novel potential virulence factors and will help to further understand the pathogenesis of A. veronii .