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Gpr126 (Adgrg6) is expressed in cell types known to be exposed to mechanical stimuli
Author(s) -
Musa Gentian,
CazorlaVázquez Salvador,
Amerongen Machteld J.,
Stemmler Marc P.,
Eckstein Markus,
Hartmann Arndt,
Braun Thomas,
Brabletz Thomas,
Engel Felix B.
Publication year - 2019
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/nyas.14135
Subject(s) - microbiology and biotechnology , biology
GPR126 (ADGRG6) is an adhesion G protein–coupled receptor that plays an important role in a variety of tissues/organs, such as heart, sciatic nerve, cartilage, and ear. Moreover, GPR126 ( ADGRG6 ) mutations are associated with human diseases, like adolescent idiopathic scoliosis, lung disease, bladder cancer, and intellectual disability. Despite its clinical importance, it remains elusive how GPR126 is activated and mediates signal transduction and what cellular processes depend on GPR126 signaling. Here, we generated a lacZ reporter mouse line to determine endogenous Gpr126 ( Adgrg6 ) expression in a cell type‐specific manner during embryonic development, at postnatal day 5 and in adult animals. Our results confirm Gpr126 expression data previously obtained utilizing antibodies and in situ hybridization in embryonic heart and sciatic nerve. In addition, we provide data with cellular resolution for previously described RT‐PCR–based data, including lung and bladder. Moreover, new Gpr126 ‐expressing tissues and cell types were identified, such as ureter and acinar secretory cells. Collectively, our data demonstrate that the newly generated lacZ reporter mouse is a suitable model to study Gpr126 expression during development and adulthood, provide detailed insight into Gpr126 expression at the cellular level, and reveal that all identified Gpr126 ‐expressing cells are known to be exposed to mechanical stimuli.