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Converting cell fates: generating hematopoietic stem cells de novo via transcription factor reprogramming
Author(s) -
Daniel Michael G.,
Lemischka Ihor R.,
Moore Kateri
Publication year - 2016
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/nyas.12989
Subject(s) - reprogramming , induced pluripotent stem cell , stem cell , microbiology and biotechnology , embryonic stem cell , biology , somatic cell , haematopoiesis , cell potency , regenerative medicine , transcription factor , induced stem cells , cellular differentiation , cell , genetics , gene
Even though all paradigms of stem cell therapy and regenerative medicine emerged from the study of hematopoietic stem cells (HSCs), the inability to generate these cells de novo or expand them in vitro persists. Initial efforts to obtain these cells began with the use of embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technologies, but these strategies have yet to yield fully functional cells. Subsequently, more recent approaches involve transcription factor (TF) overexpression to reprogram PSCs and various somatic cells. The induction of pluripotency with just four TFs by Yamanaka informs our ability to convert cell fates and demonstrates the feasibility of utilizing terminally differentiated cells to generate cells with multilineage potential. In this review, we discuss the recent efforts undertaken using TF‐based reprogramming strategies to convert several cell types into HSCs.