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The ERF‐VII transcription factor SmERF73 coordinately regulates tanshinone biosynthesis in response to stress elicitors in Salvia miltiorrhiza
Author(s) -
Zheng Han,
Jing Li,
Jiang Xihong,
Pu Chunjuan,
Zhao Shuangshuang,
Yang Jian,
Guo Juan,
Cui Guanghong,
Tang Jinfu,
Ma Ying,
Yu Muyao,
Zhou Xiuteng,
Chen Meilan,
Lai Changjiangsheng,
Huang Luqi,
Shen Ye
Publication year - 2021
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.17463
Subject(s) - salvia miltiorrhiza , jasmonic acid , transcription factor , gene silencing , gene , biology , transcription (linguistics) , microbiology and biotechnology , chemistry , biochemistry , medicine , linguistics , philosophy , alternative medicine , pathology , traditional chinese medicine
Summary Here, we investigate the role of SmERF73, a group VII ETHYLENE RESPONSE FACTOR stress response transcription factor, in the regulation of post‐modification of the skeleton precursors of diterpene tanshinones in Salvia miltiorrhiza . Most genes found to be involved in tanshinone biosynthesis are located on chromosome 6, and five of these genes comprise a large gene cluster in S. miltiorrhiza . We found that SmERF73 overexpression in S. miltiorrhiza coordinately up‐regulated the transcription of seven tanshinone biosynthetic genes, four of which were located in the tanshinone gene cluster, consequently increasing tanshinone accumulation, while SmERF73 silencing reduced corresponding gene transcription and tanshinone accumulation. SmERF73 recognizes GCC‐box promoter elements of four tanshinone‐associated genes ( DXR1 , CPS1 , KSL1 and CYP76AH3 ) and activates their expression. Moreover, SmERF73 and its targets were up‐regulated by stress elicitors; SmERF73 appears to be at least partly mediated by the jasmonic acid (JA) signaling pathway via interaction with SmJAZ3. SmERF73 coordinately regulates tanshinone biosynthetic gene expression, suggesting a potential link between tanshinone production and plant stress responses.