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A nonproteinaceous Fusarium cell wall extract triggers receptor‐like protein‐dependent immune responses in Arabidopsis and cotton
Author(s) -
Babilonia Kevin,
Wang Ping,
Liu Zunyong,
Jamieson Pierce,
Mormile Brendan,
Rodrigues Olivier,
Zhang Lin,
Lin Wenwei,
Danmaigona Clement Catherine,
Menezes de Moura Stéfanie,
AlvesFerreira Marcio,
Finlayson Scott A.,
Loring Nichols Robert,
Wheeler Terry A.,
Dever Jane K.,
Shan Libo,
He Ping
Publication year - 2021
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.17146
Subject(s) - biology , fusarium oxysporum , arabidopsis , elicitor , pseudomonas syringae , virulence , fusarium wilt , microbiology and biotechnology , plant disease resistance , pattern recognition receptor , cell wall , plant defense against herbivory , immune system , arabidopsis thaliana , pathogen , effector , botany , innate immune system , genetics , gene , mutant
SummaryFusarium wilt caused by the ascomycete fungus Fusarium oxysporum is a devastating disease of many economically important crops. The mechanisms underlying plant responses to F. oxysporum infections remain largely unknown. We demonstrate here that a water‐soluble, heat‐resistant and nonproteinaceous F. oxysporum cell wall extract (FoCWE) component from multiple F. oxysporum isolates functions as a race‐nonspecific elicitor, also termed pathogen‐associated molecular pattern (PAMP). FoCWE triggers several demonstrated immune responses, including mitogen‐activated protein (MAP) kinase phosphorylation, reactive oxygen species (ROS) burst, ethylene production, and stomatal closure, in cotton and Arabidopsis. Pretreated FoCWE protects cotton seeds against infections by virulent F. oxysporum f. sp. vasinfectum ( Fov ), and Arabidopsis plants against the virulent bacterium, Pseudomonas syringae , suggesting the potential application of FoCWEs in crop protection. Host‐mediated responses to FoCWE do not appear to require LYKs/CERK1, BAK1 or SOBIR1, which are commonly involved in PAMP perception and/or signalling. However, FoCWE responses and Fusarium resistance in cotton partially require two receptor‐like proteins, GhRLP20 and GhRLP31. Transcriptome analysis suggests that FoCWE preferentially activates cell wall‐mediated defence, and Fov has evolved virulence mechanisms to suppress FoCWE‐induced defence. These findings suggest that FoCWE is a classical PAMP that is potentially recognised by a novel pattern‐recognition receptor to regulate cotton resistance to Fusarium infections.