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white panicle 2 encoding thioredoxin z , regulates plastid RNA editing by interacting with multiple organellar RNA editing factors in rice
Author(s) -
Wang Yunlong,
Wang Yihua,
Ren Yulong,
Duan Erchao,
Zhu Xiaopin,
Hao Yuanyuan,
Zhu Jianping,
Chen Rongbo,
Lei Jie,
Teng Xuan,
Zhang Yuanyan,
Wang Di,
Zhang Xin,
Guo Xiuping,
Jiang Ling,
Liu Shijia,
Tian Yunlu,
Liu Xi,
Chen Liangming,
Wang Haiyang,
Wan Jianmin
Publication year - 2021
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.17047
Subject(s) - thioredoxin , rna editing , arabidopsis , biology , chloroplast , mutant , plastid , rna , arabidopsis thaliana , gene , genetics , microbiology and biotechnology
Summary Thioredoxins (TRXs) occur in plant chloroplasts as complex disulphide oxidoreductases. Although many biological processes are regulated by thioredoxins, the regulatory mechanism of chloroplast TRXs are largely unknown. Here we report a rice white panicle2 mutant caused by a mutation in the thioredoxin z gene, an orthologue of AtTRX z in Arabidopsis. white panicle2 ( wp2 ) seedlings exhibited a high‐temperature‐sensitive albinic phenotype. We found that plastid multiple organellar RNA editing factors (MORFs) were the regulatory targets of thioredoxin z. We showed that OsTRX z protein physically interacts with OsMORFs in a redox‐dependent manner and that the redox state of a conserved cysteine in the MORF box is essential for MORF–MORF interactions. wp2 and OsTRX z knockout lines show reduced editing efficiencies in many plastidial‐encoded genes especially under high‐temperature conditions. An Arabidopsis trx z mutant also exhibited significantly reduced chloroplast RNA editing. Our combined results suggest that thioredoxin z regulates chloroplast RNA editing in plants by controlling the redox state of MORFs.