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Retrograde signalling from the mitochondria to the nucleus translates the positive effect of ethylene on dormancy breaking of Arabidopsis thaliana seeds
Author(s) -
Jurdak Rana,
LaunayAvon Alexandra,
PaysantLe Roux Christine,
Bailly Christophe
Publication year - 2021
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.16985
Subject(s) - ethylene , germination , dormancy , reactive oxygen species , arabidopsis thaliana , microbiology and biotechnology , arabidopsis , biology , mitochondrion , seed dormancy , transcriptome , crosstalk , botany , mitochondrial ros , biochemistry , biophysics , chemistry , gene expression , gene , physics , mutant , optics , catalysis
Summary Ethylene and reactive oxygen species (ROS) regulate seed dormancy alleviation, but the molecular basis of their action and crosstalk remains largely unknown. Here we studied the mechanism of Arabidopsis seed dormancy release by ethylene using cell imaging, and genetic and transcriptomics approaches, in order to tackle its possible interaction with ROS homeostasis. We found that the effect of ethylene on seed germination required ROS production by the mitochondrial electron transport chain. Seed response to ethylene involved a mitochondrial retrograde response (MRR) through nuclear ROS production and upregulation of the MRR components AOX1a and ANAC013 , but also required the activation of the ethylene canonical pathway. Together our data allowed deciphering of the mode of action of ethylene on seed germination and the associated dynamics of ROS production. Our findings highlight the occurrence of retrograde signalling in seed germination.