Premium
RLP/K enrichment sequencing; a novel method to identify receptor‐like protein ( RLP ) and receptor‐like kinase ( RLK ) genes
Author(s) -
Lin Xiao,
Armstrong Miles,
Baker Katie,
Wouters Doret,
Visser Richard G. F.,
Wolters Pieter J.,
Hein Ingo,
Vleeshouwers Vivianne G. A. A.
Publication year - 2020
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.16608
Subject(s) - biology , genetics , gene , phytophthora infestans , immune receptor , quantitative trait locus , receptor , genome , plant disease resistance , leucine rich repeat , population , locus (genetics) , effector , candidate gene , microbiology and biotechnology , computational biology , demography , sociology
Summary The identification of immune receptors in crop plants is time‐consuming but important for disease control. Previously, resistance gene enrichment sequencing (RenSeq) was developed to accelerate mapping of nucleotide‐binding domain and leucine‐rich repeat containing ( NLR ) genes. However, resistances mediated by pattern recognition receptors (PRRs) remain less utilized. Here, our pipeline shows accelerated mapping of PRRs. Effectoromics leads to precise identification of plants with target PRRs, and subsequent RLP/K enrichment sequencing (RLP/KSeq) leads to detection of informative single nucleotide polymorphisms that are linked to the trait. Using Phytophthora infestans as a model, we identified Solanum microdontum plants that recognize the apoplastic effectors INF1 or SCR74. RLP/KSeq in a segregating Solanum population confirmed the localization of the INF1 receptor on chromosome 12, and led to the rapid mapping of the response to SCR74 to chromosome 9. By using markers obtained from RLP/KSeq in conjunction with additional markers, we fine‐mapped the SCR74 receptor to a 43‐kbp G‐LecRK locus. Our findings show that RLP/KSeq enables rapid mapping of PRRs and is especially beneficial for crop plants with large and complex genomes. This work will enable the elucidation and characterization of the nonNLR plant immune receptors and ultimately facilitate informed resistance breeding.