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Dynamic subcellular translocation of V‐type H + ‐ATPase is essential for biomineralization of the diatom silica cell wall
Author(s) -
Yee Daniel P.,
Hildebrand Mark,
Tresguerres Martin
Publication year - 2020
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.16329
Subject(s) - frustule , microbiology and biotechnology , subcellular localization , thalassiosira pseudonana , biomineralization , biochemistry , chemistry , endomembrane system , biosynthesis , biology , cytoplasm , cell , diatom , golgi apparatus , gene , botany , paleontology , phytoplankton , organic chemistry , nutrient
Summary Diatom cell walls, called frustules, are main sources of biogenic silica in the ocean and their intricate morphology is an inspiration for nanoengineering. Here we show dynamic aspects of frustule biosynthesis involving acidification of the silica deposition vesicle (SDV) by V‐type H + ATPase (VHA). Transgenic Thalassiosira pseudonana expressing the VHA B subunit tagged with enhanced green fluorescent protein (VHA B ‐eGFP) enabled subcellular protein localization in live cells. In exponentially growing cultures, VHA B ‐eGFP was present in various subcellular localizations including the cytoplasm, SDVs and vacuoles. We studied the role of VHA during frustule biosynthesis in synchronized cell cultures of T. pseudonana . During the making of new biosilica components, VHA B ‐eGFP first localized in the girdle band SDVs, and subsequently in valve SDVs. In single cell time‐lapse imaging experiments, VHA B ‐eGFP localization in SDVs precluded accumulation of the acidotropic silica biomineralization marker PDMPO. Furthermore, pharmacological VHA inhibition prevented PDMPO accumulation in the SDV, frustule biosynthesis and cell division, as well as insertion of the silicalemma‐associated protein SAP1 into the SDVs. Finally, partial inhibition of VHA activity affected the nanoscale morphology of the valve. Altogether, these results indicate that VHA is essential for frustule biosynthesis by acidifying the SDVs and regulating the insertion of other structural proteins into the SDV.