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Natural variation at Os CERK 1 regulates arbuscular mycorrhizal symbiosis in rice
Author(s) -
Huang Renliang,
Li Zheng,
Mao Cui,
Zhang Hui,
Sun Zhongfeng,
Li Hao,
Huang Congcong,
Feng Yong,
Shen Xianhua,
Bucher Marcel,
Zhang Zhongming,
Lin Yongjun,
Cao Yangrong,
Duanmu Deqiang
Publication year - 2020
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.16158
Subject(s) - colonisation , biology , cultivar , botany , rhizophagus irregularis , symbiosis , colonization , horticulture , genetics , arbuscular mycorrhizal , bacteria , microbiology and biotechnology
Summary The symbiotic interaction between arbuscular mycorrhizal fungi ( AMF ) and land plants is essential for efficient nutrient acquisition and utilisation. Our understanding of key processes controlling the AMF colonisation in rice is still limited. Dongxiang wild rice ( DY ) exhibited a stronger colonisation with Rhizophagus irregularis than the rice cultivar Zhongzao 35 ( ZZ 35). Chromosome segment substitution lines were constructed and the Os CERK 1 gene from DY was mapped. Transgenic plants in the japonica rice Zhonghua 11 ( ZZ 11) were constructed to compare root colonisation by AMF . Chromosome single‐segment substitution lines containing Os CERK 1 DY showed higher phosphorus content and grain yield relative to ZZ 35. Four amino acids substitutions were identified among the Os CERK 1 haplotypes of DY , ZZ 35 and ZH 11 and two of these were in the second lysine‐motif domain, which is essential for the differences of AMF colonisation level among rice varieties. Heterologous expression of Os CERK 1 DY in ZH 11 significantly enhanced AMF colonisation and increased resistance against the pathogenic fungi Magnaporthe oryzae . Notably, the Os CERK 1 DY haplotype was absent from 4660 cultivated rice varieties. We conclude that Os CERK 1 is a key gene affecting the symbiotic interaction with AMF and Os CERK 1 DY has the biotechnological potential to increase rice phosphorus acquisition and utilisation efficiency for sustainable agriculture.