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The complexity of intercellular localisation of alkaloids revealed by single‐cell metabolomics
Author(s) -
Yamamoto Kotaro,
Takahashi Katsutoshi,
Caputi Lorenzo,
Mizuno Hajime,
RodriguezLopez Carlos E.,
Iwasaki Tetsushi,
Ishizaki Kimitsune,
Fukaki Hidehiro,
Ohnishi Miwa,
Yamazaki Mami,
Masujima Tsutomu,
O'Connor Sarah E.,
Mimura Tetsuro
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.16138
Subject(s) - catharanthus roseus , vindoline , metabolomics , vinblastine , biology , plant cell , biochemistry , intracellular , terpenoid , vinca , chemistry , botany , bioinformatics , genetics , chemotherapy , gene
SummaryCatharanthus roseus is a medicinal plant well known for producing bioactive compounds such as vinblastine and vincristine, which are classified as terpenoid indole alkaloids ( TIA s). Although the leaves of this plant are the main source of these antitumour drugs, much remains unknown on how TIA s are biosynthesised from a central precursor, strictosidine, to various TIA s in planta . Here, we have succeeded in showing, for the first time in leaf tissue of C. roseus , cell‐specific TIA s localisation and accumulation with 10 μm spatial resolution Imaging mass spectrometry (Imaging MS) and live single‐cell mass spectrometry (single‐cell MS ). These metabolomic studies revealed that most TIA precursors (iridoids) are localised in the epidermal cells, but major TIA s including serpentine and vindoline are localised instead in idioblast cells. Interestingly, the central TIA intermediate strictosidine also accumulates in both epidermal and idioblast cells of C. roseus . Moreover, we also found that vindoline accumulation increases in laticifer cells as the leaf expands. These discoveries highlight the complexity of intercellular localisation in plant specialised metabolism.