Premium
Novel crosstalk between ethylene‐ and jasmonic acid‐pathway responses to a piercing–sucking insect in rice
Author(s) -
Ma Feilong,
Yang Xiaofang,
Shi Zhenying,
Miao Xuexia
Publication year - 2020
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.16111
Subject(s) - jasmonic acid , crosstalk , brown planthopper , oryza sativa , drosophila melanogaster , biology , signal transduction , mutant , ubiquitin ligase , insect , microbiology and biotechnology , rna interference , gene , ubiquitin , biochemistry , botany , rna , optics , physics
Summary Ethylene ( ET ) and jasmonic acid ( JA ) play important roles in plant defenses against biotic stresses. Crosstalk between JA and ET has been well studied in mediating pathogen resistance, but its roles in piercing–sucking insect resistance are unclear. The brown planthopper ( BPH ; Nilaparvata lugens ) is the most notorious piercing–sucking insect specific to rice ( Oryza sativa ) that severely affects yield. A genetic analysis revealed that Os EBF 1 and Os EIL 1 , which are in the ET signaling pathway, positively and negatively regulated BPH resistance, respectively. Molecular and biochemical analyses revealed direct interactions between Os EBF 1 and Os EIL 1. Os EBF 1, an E3 ligase, mediated the degradation of Os EIL 1 through the ubiquitination pathway, indicating the negative regulation of the ET ‐signaling pathway in response to BPH infestation. An RNA sequencing analysis revealed that a JA biosynthetic pathway‐related gene, Os LOX 9 , was downregulated significantly in the oseil1 mutant. Biochemical analyses, including yeast one‐hybrid, dual luciferase, and electrophoretic mobility shift assay, confirmed the direct regulation of Os LOX 9 by Os EIL 1. This study revealed the synergistic and negative regulation of JA and ET pathways in response to piercing–sucking insect attack. The synergistic mechanism was realized by transcriptional regulation of Os EIL 1 on Os LOX 9 . Os EIL 1 ‐ Os LOX 9 is a novel crosstalk site in these two phytohormone signaling pathways.