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The long noncoding RNA T5120 regulates nitrate response and assimilation in Arabidopsis
Author(s) -
Liu Fei,
Xu Yiran,
Chang Kexin,
Li Shuna,
Liu Zhiguang,
Qi Shengdong,
Jia Jingbo,
Zhang Min,
Crawford Nigel M.,
Wang Yong
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.16038
Subject(s) - arabidopsis , nitrate , nitrogen assimilation , biology , transcription factor , transcription (linguistics) , microbiology and biotechnology , rna , mutant , long non coding rna , gene expression , biochemistry , gene , ecology , linguistics , philosophy
Summary Long noncoding RNA s (lnc RNA s) are crucial regulators in many plant biological processes. However, it remains unknown whether lnc RNA s can respond to nitrate or function in nitrate regulation. We detected 695 lnc RNA s, 480 known and 215 novel, in Arabidopsis seedling roots; six showed altered expression in response to nitrate treatment, among which T5120 showed the highest induction. Overexpression of T5120 in Arabidopsis promoted the response to nitrate, enhanced nitrate assimilation and improved biomass and root development. Biochemical and molecular analyses revealed that NLP 7, a master nitrate regulatory transcription factor, directly bound to the nitrate‐responsive cis ‐element ( NRE )‐like motif of the T5120 promoter and activated T5120 transcription. In addition, T5120 partially restored the nitrate signalling and assimilation phenotypes of nlp7 mutant, suggesting that T5120 is involved in NLP 7‐mediated nitrate regulation. Interestingly, the expression of T5120 was regulated by the nitrate sensor NRT 1.1. Therefore, T5120 is modulated by NLP 7 and NRT 1.1 to regulate nitrate signalling. Our work reveals a new regulatory mechanism in which lnc RNA T5120 functions in nitrate regulation, providing new insights into the nitrate signalling network. Importantly, lnc RNA T5120 can promote nitrate assimilation and plant growth to improve nitrogen use efficiency.