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Phosphatase GhDs PTP 3a interacts with annexin protein Gh ANN 8b to reversely regulate salt tolerance in cotton ( Gossypium spp.)
Author(s) -
Mu Chun,
Zhou Lin,
Shan Libo,
Li Fangjun,
Li Zhaohu
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15850
Subject(s) - phosphatase , salinity , gossypium , gene silencing , ectopic expression , microbiology and biotechnology , arabidopsis , biology , phosphorylation , botany , biochemistry , gene , mutant , ecology
Summary Salinity is among the major factors limiting crop production worldwide. Despite having moderate salt‐tolerance, cotton ( Gossypium spp.) suffers severe yield losses to salinity stresses, largely due to being grown on saline‐alkali and dry lands. To identify genetic determinants conferring salinity tolerance in cotton, we deployed a functional genomic screen using a cotton cDNA library in a virus‐induced gene silencing ( VIGS ) vector. We have revealed that silencing of GhDs PTP 3a , which encodes a protein phosphatase, increases cotton tolerance to salt stress. Yeast two‐hybrid screens indicated that GhDs PTP 3a interacts with Gh ANN 8b, an annexin protein, which plays a positive role in regulating cotton response to salinity stress. Salt stress induces Gh ANN 8b phosphorylation, which is subsequently dephosphorylated by GhDs PTP 3a. Ectopic expression of GhDs PTP 3a and Gh ANN 8b oppositely regulates plant salt tolerance and calcium influx. In addition, we have revealed that silencing of GhDs PTP 3a or Gh ANN 8b exerts opposing roles in regulating Gh SOS 1 transcript levels, and ectopic expression of Gh ANN 8b elevates Na + efflux in Arabidopsis under salinity stress. Our study demonstrates that a cotton phosphatase GhDs PTP 3a and an annexin protein Gh ANN 8b interact and reversely modulate Ca 2+ and Na + fluxes in cotton salinity responses.