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Rice FLOURY ENDOSPERM 10 encodes a pentatricopeptide repeat protein that is essential for the trans ‐splicing of mitochondrial nad1 intron 1 and endosperm development
Author(s) -
Wu Mingming,
Ren Yulong,
Cai Maohong,
Wang Yunlong,
Zhu Shanshan,
Zhu Jianping,
Hao Yuanyuan,
Teng Xuan,
Zhu Xiaopin,
Jing Ruonan,
Zhang Huan,
Zhong Mingsheng,
Wang Yongfei,
Lei Cailin,
Zhang Xin,
Guo Xiuping,
Cheng Zhijun,
Lin Qibing,
Wang Jie,
Jiang Ling,
Bao Yiqun,
Wang Yihua,
Wan Jianmin
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15814
Subject(s) - endosperm , pentatricopeptide repeat , biology , rna splicing , intron , microbiology and biotechnology , mitochondrial dna , genetics , mitochondrion , gene , rna , rna editing
Summary Endosperm, the major storage organ in cereal grains, determines grain yield and quality. Despite the fact that a role for P‐type pentatricopeptide repeat ( PPR ) proteins in the regulation of endosperm development has emerged, molecular functions of many P‐type PPR proteins remain obscure. Here, we report a rice endosperm defective mutant, floury endosperm10 ( flo10 ), which developed smaller starch grains in starchy endosperm and abnormal cells in the aleurone layer. Map‐based cloning and rescued experiments showed that FLO 10 encodes a P‐type PPR protein with 26 PPR motifs, which is localized to mitochondria. Loss of function of FLO 10 affected the trans ‐splicing of the mitochondrial nad1 intron 1, which was accompanied by the increased accumulation of the nad1 exon 1 and exons 2–5 precursors. The failed formation of mature nad1 led to a dramatically decreased assembly and activity of complex I, reduced ATP production, and changed mitochondrial morphology. In addition, loss of function of FLO 10 significantly induced an alternative respiratory pathway involving alternative oxidase. These results reveal that FLO 10 plays an important role in the maintenance of mitochondrial function and endosperm development through its effect on the trans ‐splicing of the mitochondrial nad1 intron 1 in rice.