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JAZ proteins modulate seed germination through interaction with ABI 5 in bread wheat and Arabidopsis
Author(s) -
Ju Lan,
Jing Yexing,
Shi Pengtao,
Liu Jie,
Chen Jiansheng,
Yan Jijun,
Chu Jinfang,
Chen KunMing,
Sun Jiaqiang
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15757
Subject(s) - arabidopsis , abscisic acid , bimolecular fluorescence complementation , complementation , jasmonate , germination , crosstalk , microbiology and biotechnology , biology , repressor , protein fragment complementation assay , gene , chemistry , mutant , botany , biochemistry , gene expression , physics , optics
Summary Appropriate regulation of crop seed germination is of significance for agriculture production. In this study, we show that Ta JAZ 1, most closely related to Arabidopsis JAZ 3, negatively modulates abscisic acid ( ABA )‐inhibited seed germination and ABA ‐responsive gene expression in bread wheat. Biochemical interaction assays demonstrate that the C‐terminal part containing the Jas domain of Ta JAZ 1 physically interacts with Ta ABI 5. Similarly, Arabidopsis jasmonate‐ZIM domain ( JAZ ) proteins also negatively modulate ABA responses. Further we find that a subset of JAZ proteins could interact with ABI 5 using the luciferase complementation imaging assays. Choosing JAZ 3 as a representative, we demonstrate that JAZ 3 interacts with ABI 5 in vivo and represses the transcriptional activation activity of ABI 5. ABA application could abolish the enrichment of JAZ proteins in the ABA ‐responsive gene promoter. Furthermore, we find that ABA application could induce the expression of jasmonate ( JA ) biosynthetic genes and then increase the JA concentrations partially dependent on the function of ABI 5, consequently leading to the degradation of JAZ proteins. This study sheds new light on the crosstalk between JA and ABA in modulating seed germination in bread wheat and Arabidopsis .