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Plant pathogenic fungi Colletotrichum and Magnaporthe share a common G 1 phase monitoring strategy for proper appressorium development
Author(s) -
Fukada Fumi,
Kodama Sayo,
Nishiuchi Takumi,
Kajikawa Naoki,
Kubo Yasuyuki
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15728
Subject(s) - appressorium , biology , magnaporthe , colletotrichum , cytokinesis , virulence , septin , fungal protein , microbiology and biotechnology , mutant , gene , genetics , botany , cell division , cell , oryza sativa , magnaporthe grisea
Summary To breach the plant cuticle, many plant pathogenic fungi differentiate specialized infection structures (appressoria). In Colletotrichum orbiculare (cucumber anthracnose fungus), this differentiation requires unique proper G 1 /S phase progression, regulated by two‐component GTPase activating protein CoBub2/CoBfa1 and GTP ase CoTem1. Since their homologues regulate mitotic exit, cytokinesis, or septum formation from yeasts to mammals, we asked whether the BUB 2 function in G 1 /S progression is specific to plant pathogenic fungi. Colletotrichum higginsianum and Magnaporthe oryzae were genetically analyzed to investigate conservation of BUB 2 roles in cell cycle regulation, septum formation, and virulence. Expression profile of cobub2Δ was analyzed using a custom microarray. In bub2 mutants of both fungi, S phase initiation was earlier, and septum formation coordinated with a septation initiation network protein and contractile actin ring was impaired. Earlier G 1 /S transition in cobub2Δ results in especially high expression of DNA replication genes and differing regulation of virulence‐associated genes that encode proteins such as carbohydrate‐active enzymes and small secreted proteins. The virulence of chbub2Δ and mobub2Δ was significantly reduced. Our evidence shows that BUB 2 regulation of G 1 /S transition and septum formation supports its specific requirement for appressorium development in plant pathogenic fungi.