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Protein and metabolite composition of Arabidopsis stress granules
Author(s) -
Kosmacz Monika,
Gorka Michał,
Schmidt Stephan,
Luzarowski Marcin,
Moreno Juan C.,
Szlachetko Jagoda,
Leniak Ewa,
Sokolowska Ewelina M.,
Sofroni Kostika,
Schnittger Arp,
Skirycz Aleksandra
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15690
Subject(s) - arabidopsis , stress granule , biology , proteomics , proteome , biochemistry , microbiology and biotechnology , metabolome , chemistry , metabolite , messenger rna , gene , translation (biology) , mutant
Summary Stress granules ( SG s) are evolutionary conserved aggregates of proteins and untranslated mRNA s formed in response to stress. Despite their importance for stress adaptation, no complete proteome composition has been reported for plant SG s. In this study, we addressed the existing gap. Importantly, we also provide evidence for metabolite sequestration within the SG s. To isolate SG s we used Arabidopsis seedlings expressing green fluorescent protein ( GFP) fusion of the SG s marker protein, Rbp47b, and an experimental protocol combining differential centrifugation with affinity purification (AP). SG s isolates were analysed using mass spectrometry‐based proteomics and metabolomics. A quarter of the identified proteins constituted known or predicted SG components. Intriguingly, the remaining proteins were enriched in key enzymes and regulators, such as cyclin‐dependent kinase A ( CDKA ), that mediate plant responses to stress. In addition to proteins, nucleotides, amino acids and phospholipids also accumulated in SG s. Taken together, our results indicated the presence of a preexisting SG protein interaction network; an evolutionary conservation of the proteins involved in SG assembly and dynamics; an important role for SG s in moderation of stress responses by selective storage of proteins and metabolites.