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Os HOP 2 regulates the maturation of crossovers by promoting homologous pairing and synapsis in rice meiosis
Author(s) -
Shi Wenqing,
Tang Ding,
Shen Yi,
Xue Zhihui,
Zhang Fanfan,
Zhang Chao,
Ren Lijun,
Liu Changzhen,
Du Guijie,
Li Yafei,
Yan Changjie,
Cheng Zhukuan
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15664
Subject(s) - synapsis , synaptonemal complex , homologous chromosome , meiosis , biology , meiocyte , genetics , microbiology and biotechnology , homologous recombination , pairing , gene , physics , superconductivity , quantum mechanics
Summary Meiotic recombination is closely linked with homologous pairing and synapsis. Previous studies have shown that HOMOLOGOUS PAIRING PROTEIN2 (HOP2), plays an essential role in homologous pairing and synapsis. However, the mechanism by which HOP 2 regulates crossover ( CO ) formation has not been elucidated. Here, we show that Os HOP 2 mediates the maturation of CO s by promoting homologous pairing and synapsis in rice ( Oryza sativa ) meiosis. We used a combination of genetic analysis, immunolocalization and super‐resolution imaging to analyze the function of Os HOP 2 in rice meiosis. We showed that full‐length pairing, synapsis and CO formation are disturbed in Oshop2 meiocytes. Moreover, structured illumination microscopy showed that Os HOP 2 localized to chromatin and displayed considerable co‐localization with axial elements ( AE s) and central elements ( CE s). Importantly, the interaction between Os HOP 2 and a transverse filament protein of synaptonemal complex (ZEP1), provided further evidence that Os HOP 2 was involved in assembly or stabilization of the structure of the synaptonemal complex ( SC ). Although the initiation of recombination and CO designation occur normally in Oshop2 mutants, mature CO s were severely reduced, and human enhancer of invasion 10 (HEI10)10 foci were only present on the synapsed region. Putting the data together, we speculate that Os HOP 2 may serve as a global regulator to coordinate homologous pairing, synapsis and meiotic recombination in rice meiosis.