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Cotton fiber elongation requires the transcription factor Gh MYB 212 to regulate sucrose transportation into expanding fibers
Author(s) -
Sun Wenjie,
Gao Zhengyin,
Wang Jun,
Huang Yiqun,
Chen Yun,
Li Jianfu,
Lv Mengli,
Wang Jin,
Luo Ming,
Zuo Kaijing
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15620
Subject(s) - sucrose , ovule , microbiology and biotechnology , elongation , myb , fiber , sugar , sucrose synthase , starch , biology , transcription factor , chemistry , biochemistry , gene , embryo , materials science , metallurgy , ultimate tensile strength , organic chemistry , invertase
Summary Cotton is white gold across the globe and composed of fiber cells derived from the outer integument of cotton ovules. Fiber elongation uses sucrose as a direct carbon source. The molecular mechanism transcriptionally controlling sucrose transport from ovules into the elongating fibers remains elusive. In this study the involvement of Gh MYB 212 in the regulation of sucrose transportion into expanding fibers was investigated. Gh MYB 212 RNA i plants ( Gh MYB 212i ) accumulated less sucrose and glucose in developing fibers, and had shorter fibers and a lower lint index. RNA ‐seq and protein− DNA binding assays revealed that Gh MYB 212 was closely linked to the pathways of sucrose and starch transportation and metabolism, directly controling the expression of a sucrose transporter gene Gh SWEET 12 . Gh SWEET 12 RNA i plants ( Gh SWEET 12i ) possessed similar fiber phenotypes to those of Gh MYB 212i . Exogenous sucrose supplementation in ovule cultures did not rescue the shorter fiber phenotype of Gh MYB 212i and Gh SWEET 12i . This finding supported the idea that the attenuated rate of sucrose transport from the outer seed coat into the fibers is responsible for the retardation of fiber elongation. Current investigations support the idea that Gh MYB 212 functions as the main regulator of fiber elongation by controlling the expression of Gh SWEET 12 , and therefore it is important to study cell expansion and sugar transportation during seed development.