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The Sn RK 1‐ eIF iso4G1 signaling relay regulates the translation of specific mRNA s in Arabidopsis under submergence
Author(s) -
Cho HsingYi,
Lu MeiYeh Jade,
Shih MingChe
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15589
Subject(s) - arabidopsis , biology , translation (biology) , messenger rna , mutant , polysome , microbiology and biotechnology , initiation factor , rna , phosphorylation , gene , biochemistry , ribosome
Summary Cellular responses to oxygen deprivation are essential for survival during energy crises in plants and animals. Hypoxia caused by submergence results in reprogramming of translation dynamic in plants, but the molecular mechanisms are not well understood. Here we show that Arabidopsis Snf1‐related protein kinase 1 (Sn RK 1) phosphorylates the translation initiation factor eIF iso4G to regulate translation dynamic under submergence. In Arabidopsis, there are two eIF iso4G genes, eIF iso4G1 and eIF iso4G2, which belong to the eIF 4G family. Both eIF iso4Gs were phosphorylated by Sn RK 1 under submergence. Interestingly, the eIF iso4G1 knockout mutant, but not the eIF iso4G2 mutant, became more sensitive to submergence, implying that eIF iso4G1 is involved in regulating submergence tolerance in Arabidopsis. Comparison of RNA sequences in the polysome fraction and the RNA s immunoprecipitated by eIF iso4G1 from Col‐0 and the Sn RK 1 and eIF iso4G1 mutants revealed that lack of eIF iso4G1 phosphorylation disrupts the translation of specific mRNA s under submergence. Taken together, our findings suggest that the Sn RK 1‐ eIF iso4G1 relay controls the translation of an array of genes under hypoxia, including core hypoxia response genes and genes related to stress response and biosynthetic process.