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A nodule‐localized phosphate transporter Gm PT 7 plays an important role in enhancing symbiotic N 2 fixation and yield in soybean
Author(s) -
Chen Liyu,
Qin Lu,
Zhou Lili,
Li Xinxin,
Chen Zhichang,
Sun Lili,
Wang Wenfei,
Lin Zhihao,
Zhao Jing,
Yamaji Naoki,
Ma Jian Feng,
Gu Mian,
Xu Guohua,
Liao Hong
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15541
Subject(s) - nitrogen fixation , root nodule , biology , phosphate , rhizosphere , shoot , biochemistry , fixation (population genetics) , botany , microbiology and biotechnology , gene , bacteria , genetics
Summary Symbiotic nitrogen (N 2 ) fixation plays a vital role in sustainable agriculture. Efficient N 2 fixation requires various materials, including phosphate (Pi); however, the molecular mechanism underlying the transport of Pi into nodules and bacteroids remains largely unknown. A nodule‐localized Pi transporter, Gm PT 7, was functionally characterized in soybean ( Glycine max ) and its role in N 2 fixation and yield was investigated via composite and whole transgenic plants. Gm PT 7 protein was localized to the plasma membrane and showed transport activity for Pi in yeast. Altered expression of Gm PT 7 changed 33 Pi uptake from rhizosphere and translocation to bacteroids. Gm PT 7 was mainly localized to the outer cortex and fixation zones of the nodules. Overexpression of Gm PT 7 promoted nodulation, and increased plant biomass, shoot nitrogen and phosphorus content, resulting in improved soybean yield by up to 36%. Double suppression of Gm PT 5 and Gm PT 7 led to nearly complete elimination of nodulation and over 50% reduction in plant biomass, shoot nitrogen and phosphorus content, indicating that both Gm PT 7 and Gm PT 5 contribute to Pi transport for N 2 fixation. Taken together, our results indicate that Gm PT 7 is a transporter responsible for direct Pi entry to nodules and further to fixation zones, which is required for enhancing symbiotic N 2 fixation and grain yield of soybean.