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The RNA editing factor DUA 1 is crucial to chloroplast development at low temperature in rice
Author(s) -
Cui Xuean,
Wang Yanwei,
Wu Jinxia,
Han Xiao,
Gu Xiaofeng,
Lu Tiegang,
Zhang Zhiguo
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15448
Subject(s) - pentatricopeptide repeat , biology , chloroplast , rna editing , rna , genetics , germplasm , cultivar , microbiology and biotechnology , botany , gene
Summary Low temperature stress hinders plant growth and chloroplast development and can limit the geographic range of cultivars. In rice, japonica cultivars have greater chilling tolerance than indica cultivars, but the molecular mechanism underlying chilling tolerance is unclear. Here, we report an RNA ‐binding protein, DUA 1, cloned from the indica cultivar Dular , which exhibits a deficiency in chloroplast development at an early stage of development under low‐temperature conditions. DUA 1 shares high sequence homology with the pentatricopeptide repeat family and functions in plastid RNA editing under low‐temperature conditions. Our data suggest that DUA 1 can bind to the plastid‐encoded rps 8‐182 transcript and disruption of DUA 1 activity impairs editing. The RNA editing cofactor WSP 1, a partner of DUA 1, also participates in chloroplast development at low temperature. Western blot analysis indicates that WSP 1 enhances DUA 1 stability under low temperatures. DUA 1 sequence analyses of rice core germplasm revealed that three major haplotypes of DUA 1 and one haplotype showed substantial differences in chlorophyll content under low‐temperature conditions. Variation at DUA 1 may play an important role in the adaptation of rice to different growing regions.