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EFFECTOR OF TRANSCRIPTION factors are novel plant‐specific regulators associated with genomic DNA methylation in Arabidopsis
Author(s) -
Tedeschi Francesca,
Rizzo Paride,
Huong Bui Thi Mai,
Czihal Andreas,
Rutten Twan,
Altschmied Lothar,
Scharfenberg Sarah,
Grosse Ivo,
Becker Claude,
Weigel Detlef,
Bäumlein Helmut,
Kuhlmann Markus
Publication year - 2019
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.15439
Subject(s) - dna methylation , biology , transcriptome , arabidopsis , epigenetics , genetics , effector , dna , gene , transcription factor , mutant , arabidopsis thaliana , methylation , microbiology and biotechnology , gene expression
Summary Plant‐specific EFFECTORS OF TRANSCRIPTION ( ET ) are characterised by a variable number of highly conserved ET repeats, which are involved in zinc and DNA binding. In addition, ET s share a GIY ‐ YIG domain, involved in DNA nicking activity. It was hypothesised that ET s might act as epigenetic regulators. Here, methylome, transcriptome and phenotypic analyses were performed to investigate the role of ET factors and their involvement in DNA methylation in Arabidopsis thaliana . Comparative DNA methylation and transcriptome analyses in flowers and seedlings of et mutants revealed ET ‐specific differentially expressed genes and mostly independently characteristic, ET ‐specific differentially methylated regions. Loss of ET function results in pleiotropic developmental defects. The accumulation of cyclobutane pyrimidine dimers after ultraviolet stress in et mutants suggests an ET function in DNA repair.